IDENTIFICATION OF ANTIFUNGAL COMPOUNDS FROM LEAF EXTRACT OF EUCALYPTUS CITRIODORA AGAINST ASCOCHYTA RABIEI

Ascochyta rabiei (Pass.) Lab. is a highly destructive pathogen of chickpea. In thisstudy, leaf extract Eucalyptus citriodora was assessed against this pathogen. Bioassays withmethanolic (0, 05, 1.0, 1.5, …, 4.0%) revealed the remarkable antifungal potential ofleaf where 69–94% reduction in biomass A. recorded. Chloroform fraction thisextract separated by partitioning separating funnel. Using solvent system ofchloroform: n-hexane (20:80), three compounds were detected on TLC plate which separatedthrough preparative and purified HPLC. GC-MS lead to theidentification 3-cyclohexene- 1-ol, 4-methyl-1-(1-methylethyl)- (1), 1-cyclohexene- 1carboxaldehyde,4-(1-methylethyl)- (2) eucalyptol (3) that might caused activity extract.